Biological Applications of Infrared Spectroscopy
Analytical Chemistry by Open Learning
1. Auflage Juli 1997
XX, 192 Seiten, Softcover
Wiley & Sons Ltd
Kurzbeschreibung
Diese Einführung in die Grundlagen der Infrarot-Spektroskopie ist besonders für Biologen und Biochemiker gedacht. Von den theoretischen Grundlagen über experimentelle Methoden und instrumentelle Ausrüstung bis hin zur Interpretation von Spektren biologisch relevanter Spezies (Proteine, Peptide, Lipide, Nucleinsäuren, Membranen) wird das Gebiet umfassend und auch für Nichtspezialisten verständlich behandelt.
Infrared spectroscopy (IR) is a well established analytical technique for the identification of organic molecules. In this first dedicated volume, the theory of IR is described and is then related to various biological systems. Chapters on instrumentation, sample preparation and the interpretation of spectra give the reader practical help in using the technique. A comprehensive applications chapter illustrates the diversity and power of this technique in real systems.
Introduction: Electromagnetic Radiation; Distribution of Lifetimes of Energy States; Molecular Vibrations; Instrumentation: Representation of Spectra; Dispersive Spectrometers; Fourier Transform Spectrometers; Sampling Methods: Transmission methods; Reflectance Methods; Microsampling Methods; Gas Chromatography; Temperature Studies; Photoacoustic Spectroscopy; Spectrum Interpretation: Group Frequencies; Summary of Characteristic Frequencies; Aldehydes and Ketones; Complicating Factors; Interpretation of Unknown Compounds; Quantitative Analysis: Spectrum Manipulation; Pathlength Calibration; Determination of Concentration; Simple Analysis; Multicomponent Analysis; Applications: Proteins and Peptides; Lipids; Biomembranes; Animal Tissue; Carbohydrates; Pharmaceuticals; Foods; Plant Material.
