Carrier-bound Immobilized Enzymes

Principles, Application and Design

Cao, Linqiu

1. Auflage September 2005
XV, 563 Seiten, Hardcover
248 Abbildungen
67 Tabellen
Handbuch/Nachschlagewerk

ISBN: 978-3-527-31232-0

Wiley-VCH, Weinheim

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Kurzbeschreibung

The authoritative reference for the developer and user of carrier-bound enzymes covers all types of enzyme immobilization methods and supports, including novel functional supports and new strategies, such as enzyme entrapment and encapsulation.

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The first systematic overview of this key technique since the early 1990s, this authoritative reference is the only handbook available to include all recent developments. The author draws on his wide-ranging experience in both academia and industry to systematically cover all types of enzyme immobilization methods, such as adsorption-based and covalent immobilization, as well as enzyme entrapment and encapsulation. Throughout, a careful review of materials and techniques for the generation of functional immobilized enzymes benefits both developers and users of carrier-bound enzymes.
A must for biotechnologists, biochemists and preparative chemists using enzymes in their daily work.

IMMOBILIZED ENZYMES: PAST, PRESENT AND PROSPECTS
Introduction
The Past
Implications from the Past
Prospective and Future Development

ADSORPTION-BASED IMMOBILIZATION
Introduction and Classification
Principles of Adsorptive Enzyme Immobilization
Requirements of the Carriers
Factors which Dictate Enzyme Performance
Preparation of Immobilized Enzymes by Adsorption

COVALENT ENZYME IMMOBILIZATION
Introduction
Physical Nature of Carriers
Chemical Nature of Carriers
Amino Acid Residues for Covalent Binding of Enzymes
Factors Influencing Enzyme Performance
Preparation of Active Carriers

ENZYME ENTRAPMENT
Introduction and Definition
Requirements of the Carriers
Effect of the Entrapment
Preparation of Entrapped Enzymes

ENZYME ENCAPSULATION
Introduction and Classification
Effect of Encapsulation
Preparation of Encapsulated Enzymes

NON-CONVENTIONAL ENZYME IMMOBILIZATION
Coating-based Immobilization
Site-specific Immobilization
Immobilization in Organic Solvents
Imprinting Immobilization
Modification-based Immobilization
Post-immobilization Techniques

"Es wird dazu beitragen, der rationalen Kombination der vorhandenen Prinzipien zur Enzymimmobilisierung die Zukunft zu ebnen."
Nachrichten aus der Chemie

Linqiu Cao studied Organic Chemistry and Biotechnology at the Beijing University of Science and Technology (China). He was a visiting scientist at GBF (Gesellschaft für Biotechnologische Forschung) in Braunschweig (Germany) and later moved to the University of Stuttgart (Germany) to obtain a PhD in Biotechnology. He then joined the group of Roger Sheldon at the Technical University in Delft (The Netherlands), where he pioneered a novel enzyme immobilisation technology with improved performance in biocatalytic transformations using cross-linked enzyme aggregates (CLEAs). In 2000, he joined the newly founded company Avantium in Amsterdam (The Netherlands) where he continued to develop novel enzyme crosslinking and immobilisation technology. Recently he moved to DMV in Veghel (The Netherlands).

L. Cao, DMV International, Wageningen, The Netherlands