Protein Purification
Principles, High Resolution Methods, and Applications
Methods of Biochemical Analysis (Band Nr. 54)
3. Auflage April 2011
544 Seiten, Hardcover
Handbuch/Nachschlagewerk
Kurzbeschreibung
This thoroughly revised and updated third edition provides an overview of the principles and methodology of the most frequently used biochemical protein separation techniques used today. Balancing theory, procedures, and applications, this comprehensive resource is indispensable to professionals and students.
This thoroughly revised and updated third edition provides an overview of the principles and methodology of the most frequently used biochemical separation techniques for proteins used today. Balancing theory, procedures, and applications, it offers professionals and students in biochemistry, organic chemistry, and analytical chemistry quick access to a wide range of important techniques, including:
* Gel filtration and ion exchange chromatography
* Affinity and covalent chromatography
* Reversed phase and hydrophobic interaction chromatography
* Ligands from combinatorial libraries
* Electrophoresis in gels
* Immunoelectrophoresis and capillary electrophoresis
* Two-dimensional electrophoresis in proteomics
* Protein elution and blotting techniques
* Large-scale electrophoresis processes
Contributors.
Part I. Introduction.
1.Introduction to Protein Purification (Bo Ersson, Lars Rydén and Jan-Christer Janson).
Part II. Chromatography.
2. Introduction to Chromatography (Jan-Christer Janson and Jan-Åke Jönsson).
3. Gel Filtration (Lars Hagel).
4. Ion Exchange Chromatography (Evert Karlsson and Irwin Hirsh).
5. High-Resolution Reversed-Phase Chromatography (Sylvia Winkel Pettersson).
6. Hydrophobic Interaction Chromatography (Kjell-Ove Eriksson and Makonnen Belew).
7. Immobilized Metal Ion Affinity Chromatography (Lennart Kågedal).
8. Covalent Chromatpgraphy (Francisco Batista-Viera, Lars Rydén and Jan Carlsson).
9. Affinity Chromatography (Francisco Batista-Viera, Jan-Christer Janson and Jan Carlsson).
10. Affinity Ligands from Chemical Combinatorial Libraries (Enrique Carredano and Herbert Baumann).
11. Affinity Ligands from Biological Combinatorial Libraries (Per-Åke Nygren).
Part III. Other Separation Methods and Related Techniques.
12. Membrane Separations (Joachim K. Walter, Zuwei Jin, Maik W. Jornitz and Uwe Gottschalk).
13. Refolding of Inclusion Body Proteins from E. coli (Zhiguo Su, Siannan Lu and Zheng Liu).
14. Purification of PEGylated Proteins (Conan J. Fee and James M. Van Alstine).
Part IV. Electrophoresis.
15. Electrophoresis in Gels (Reiner Westermeier).
16. Conventional Isoelectric Focusing in Gel Slabs and Capillaries and Immobilized pH Gradients (Pier Giorgio Righetti, Alessia Farinazzo, Elisa Fasoli and Sabina Carla Righetti).
17. Two-Dimensional Electrophoresis in Proteomics (Reiner Westermeier and Angelika Görg).
18. Protein Elution and Blotting Techniques (Reiner Westermeier).
19. Capillary Electrophoretic Separations (Wolfgang Thormann).
Part V. Separation Method Optimization.
20. High Throughput Screening Techniques on Protein Purification (Karol M. Lacki and Eggert Brekkan).
Index.
