Sample Preparation in LC-MS Bioanalysis
Wiley Series on Pharmaceutical Science
1. Auflage April 2019
384 Seiten, Hardcover
Wiley & Sons Ltd
Revised and Expanded Handbook Provides Comprehensive Introduction and Complete Instruction for Sample Preparation in Vital Category of Bioanalysis
Following in the footsteps of the previously published Handbook of LC-MS Bioanalysis, this book is a thorough and timely guide to all important sample preparation techniques used for quantitative Liquid Chromatography-Mass Spectrometry (LC-MS) bioanalysis of small and large molecules. LC-MS bioanalysis is a key element of pharmaceutical research and development, post-approval therapeutic drug monitoring, and many other studies used in human healthcare.
While advances are continually being made in key aspects of LC-MS bioanalysis such as sensitivity and throughput, the value of research/study mentioned above is still heavily dependent on the availability of high-quality data, for which sample preparation plays the critical role. Thus, this text provides researchers in industry, academia, and regulatory agencies with detailed sample preparation techniques and step-by-step protocols on proper extraction of various analyte(s) of interest from biological samples for LC-MS quantification, in accordance with current health authority regulations and industry best practices. The three sections of the book with a total of 26 chapters cover topics that include:
* Current basic sample preparation techniques (e.g., protein precipitation, liquid-liquid extraction, solid-phase extraction, salting-out assisted liquid-liquid extraction, ultracentrifugation and ultrafiltration, microsampling, sample extraction via electromembranes)
* Sample preparation techniques for uncommon biological matrices (e.g., tissues, hair, skin, nails, bones, mononuclear cells, cerebrospinal fluid, aqueous humor)
* Crucial aspects of LC-MS bioanalytical method development (e.g., pre-analytical considerations, derivation strategies, stability, non-specific binding) in addition to sample preparation techniques for challenging molecules (e.g., lipids, peptides, proteins, oligonucleotides, antibody-drug conjugates)
Sample Preparation in LC-MS Bioanalysis will prove a practical and highly valuable addition to the reference shelves of scientists and related professionals in a variety of fields, including pharmaceutical and biomedical research, mass spectrometry, and analytical chemistry, as well as practitioners in clinical pharmacology, toxicology, and therapeutic drug monitoring.
Preface
List of Abbreviations
Part I: Current sample preparation techniques in LC-MS bioanalysis
1 Basic sample preparation techniques in LC-MS bioanalysis -Protein precipitation, liquid-liquid extraction and solid phase extraction
Wenkui Li, Wenying Jian and Yunlin Fu
2 On-line extraction and column switching techniques in LC-MS bioanalysis
Yan Mao and Mike (Qingtao) Huang
3 Equilibrium dialysis, ultracentrifugation and ultrafiltration in LC-MS bioanalysis
Theo de Boer and Henri Meijering
4 Phospholipids depletion techniques in LC-MS bioanalysis
Stacy Brown and Jennifer Carmical
5 Salting-out assisted liquid-liquid extraction (SALLE) in LC-MS bioanalysis
Jun Zhang and Xin Xiong
6 Supported liquid extraction (SLE) in LC-MS bioanalysis
Zhongzhe Cheng and Hongliang Jiang
7 Immunocapture in LC-MS bioanalysis
Ang Liu
8 Microextraction techniques in LC-MS bioanalysis
Márcio Rodrigues, Ana Fortuna, Amílcar Falcão and Gilberto Alves
9 Microsampling application with LC-MS bioanalysis
Chester L Bowen and Matthew Barfield
10 Nanomaterials for sample preparation in LC-MS bioanalysis
R.A. González-Fuenzalida, N. Jornet-Martinez, R. Herráez-Hernández and P. Campíns-Falcó
11 Sample preparation via molecularly imprinted polymers (MIP) in LC-MS bioanalysis
Myriam Díaz-Álvarez and Antonio Martín-Esteban
12 Stir bar sorptive extraction for sample preparation in LC-MS bioanalysis
Maria Kechagia, Maria Kissoudi and Victoria F. Samanidou
13 Monolithic spin column extraction in LC-MS Bioanalysis
Akira Namera and Takeshi Saito
14 Aptamer-based sample preparation in LC-MS bioanalysis
Fuyou Du, Zongyu Wei, Qiulian Zeng and Guihua Ruan
15 Sample extraction via electromembrane in LC-MS bioanalysis
María Ramos-Payán, Rut Fernández-Torres and Miguel Ángel Bello-López
Part II: Matrix specific sample preparation techniques in LC-MS bioanalysis
16 Tissue sample preparation in LC-MS bioanalysis
Hong Gao and John Williams
17 Sample preparation for LC-MS bioanalysis of peripheral blood mononuclear cells
Yunlin Fu and Wenkui Li
18 Sample preparation for LC-MS bioanalysis of urine, cerebrospinal fluid, synovial fluid, sweat, tears, and aqueous humor samples
Alena Ji
19 Sample preparation for LC-MS bioanalysis of liposomal samples
Wenying Jian, Rao N.V. S Mamidi and Naidong Weng
Part III: Sample preparation techniques for LC-MS bioanalysis of challenging molecules
20 Key pre-analytical considerations in LC-MS bioanalysis
Fumin Li, Bruce Hidy and Rand Jerkins
21 Derivatization in sample preparation for LC-MS bioanalysis
Dafang Zhong and Yunting Zhu
22 Sample preparation for LC-MS bioanalysis of lipids
Samuel Hofbauer, Ashkan Salamatipour, Ian A. Blair and Clementina Mesaros
23 Sample preparation for LC-MS bioanalysis of peptides
Long Yuan
24 Sample preparation for LC-MS bioanalysis of proteins
Nico van de Merbel
25 Sample preparation for LC-MS bioanalysis of oligonucleotides
Michael G. Bartlett, Jaeah Kim, Babak Basiri and Ning Li
26 Sample preparation for LC-MS bioanalysis of anti-drug conjugates (ADCs)
Cong Wei and and Ragu Ramanathan
WENYING JIAN is a Senior Principal Scientist in Janssen Research and Development at the Johnson & Johnson Company. She serves on the Editorial Board of Journal of Pharmacological and Toxicological Methods.
YUNLIN FU is an Investigator at the Novartis Institutes for BioMedical Research Pharmacokinetic Sciences.
