Fluorescence Microscopy

Introduction to Optics
(Rainer Heintzmann and Ulrich Kubitscheck)
Principles of Light Microscopy
(Ulrich Kubitscheck)
Fluorescence Microscopy
(Jurek W. Dobrucki and Ulrich Kubitscheck)
Fluorescence Labeling
(Gerd Ulrich Nienhaus and Karin Nienhaus)
Confocal Microscopy
(Nikolaus Naredi-Rainer, Jens Prescher, Achim Hartschuh and Don C. Lamb)
Two-Photon Excitation Microscopy for Three-Dimensional Imaging of Living Intact Tissues
(David W. Piston)
Light Sheet Microscopy
(Gopi Shah, Michael Weber and Jan Huisken)
Localization-Based Super-Resolution Microscopy
(Markus Sauer and Mike Heilemann)
Super-Resolution Microscopy: Interference and Pattern Techniques
(Udo Birk, Gerrit Best, Roman Amberger and Christoph)
STED Microscopy
(Travis J. Gould, Lena K. Schroeder, Patrina A. Pellett and Joerg Bewersdorf)
Fluorescence Photobleaching Techniques
(Reiner Peters)
Single-Molecule Microscopy in the Life Sciences
(Markus Axmann, Josef Madl and Gerhard J. Schütz)
Förster Resonance Energy Transfer and Fluorescence Lifetime Imaging
(Fred S. Wouters)
Appendix A: What Exactly is a Digital Image?
Ulrich Kubitscheck
Appendix B: Practical Guide to Optical Alignment
(Rainer Heintzmann)
Index

Ulrich Kubitscheck is the department head of Biophysical Chemistry at the Institute of Physical and Theoretical Chemistry at the University of Bonn, Germany. Having obtained his academic degrees from the University of Bremen, Germany, he spent his career working at The Weizmann Institute of Science and the Institute of Medical Physics and Biophysics of the University of Münster, Germany, before taking up his present appointment at the University of Bonn. Professor Kubitscheck develops single molecule and light sheet imaging techniques, has authored over 80 scientific publications and has extensive experience in teaching courses on physical chemistry, biophysics and quantitative microscopy.