Enzyme Assays

High-throughput Screening, Genetic Selection and Fingerprinting

Reymond, Jean-Louis (Editor)

1. Edition November 2005
XVIII, 368 Pages, Hardcover
162 Pictures (6 Colored Figures)
12 tables
Handbook/Reference Book

ISBN: 978-3-527-31095-1

Wiley-VCH, Weinheim

Wiley Online Library Sample Chapter

Short Description

Enzyme assays play an essential role in biochemistry and biotechnology. This book presents the most important methods for high-throughput screenings and functional characterization of enzymes. Edited by a leading expert, it is an indispensable tool for researchers in the field.

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Edited by one of the leading experts in the field, this book fills the need for a book presenting the most important methods for high-throughput screenings and functional characterization of enzymes. It adopts an interdisciplinary approach, making it indispensable for all those involved in this expanding field, and reflects the major advances made over the past few years.
For biochemists, analytical, organic and catalytic chemists, and biotechnologists.

PART I. THE CHEMISTRY OF ENZYME ASSAYS
1. Chromogenic Assay of Hydrolases for Activity and Enantioselectivity
2. High-throughput screening systems for assaying the enantioselectivity of enzymes
3. High throughput screening methods developed for oxidoreductases
4. Industrial perspective on assays
PART II. ENZYME ASSAYS AND GENETIC SELECTION
5. Agar-plate based assays
6. Genetic selection for biocatalysts
7. Three-hybrid systems and in vivo selection
8. Nucleic-acid based approaches on the screening of enzymes.
PART III. ENZYME PROFILING
9. Fluorescent probes for lipolytic enzymes
10. Enzyme fingerprinting
11. Protease profiling
12. Enzyme assays on chips
INDEX

"...an excellent overview presenting a unique exposition of all important developments, techniques and concepts in this rapidly advancing field. [...] In summary, this book gives an excellent overview of current and relevant approaches where there is a need for enzyme assay development and use, and should be of interest to a broad readership. It is an indispensable source of information and inspiration for everyone working in the field and can highly be recommended."
ChemBioChem

"...the value of this volume to students or investigators lies in the ability to develop assays in one's own laboratory based on the material presented...The broad and diverse scope of this volume makes it best suited to be an acquisition by an organization's library." Clinical Chemistry

"....is a useful source of information, inspiration, and references for its readers across chemistry and biology."
International Journal of Biological Macromolecules

1985 Jean-Louis Reymond obtained his diploma working with Professor Seebach at the ETH Zürich. He finished his thesis in the field of total synthesis in 1989 in the group of Professor Vogel. After being in the United States for two years at the Scripps Research Institute he became an assistant professor there. 1997 he returned to the University of Bern, Switzerland. His research interest is biocatalysis and in particular enzyme design, assays, profiling, fingerprints and inhibitors.

J.-L. Reymond, Department of Chemistry and Biochemistry, University of Bern, Switzerland