Current Protocols Essential Laboratory Techniques
The latest title from the acclaimed Current Protocols series, Current Protocols Essential Laboratory Techniques, 2e provides the new researcher with the skills and understanding of the fundamental laboratory procedures necessary to run successful experiments, solve problems, and become a productive member of the modern life science laboratory. From covering the basic skills such as measurement, preparation of reagents and use of basic instrumentation to the more advanced techniques such as blotting, chromatography and real-time PCR, this book will serve as a practical reference manual for any life science researcher. Written by a combination of distinguished investigators and outstanding faculty, Current Protocols Essential Laboratory Techniques, 2e is the cornerstone on which the beginning scientist can develop the skills for a successful research career.
Foreword
Preface
Contributors List
Conversion Factors
Flow Chart: Application of Techniques
Emily Wiley, Claremont Colleges
Chapter 1 Volume/Weight Measurement
Unit 1.1 Volume Measurement
Andrew Zanella and Tom Davis, Claremont Colleges
Unit 1.2 Weight Measurement
Michael Guzy, Ohaus, Corp.
Chapter 2 Concentration Measurement
Unit 2.1 Spectrophotometry
Rob Morris, Ocean Optics, Inc.
Unit 2.2 Quantitation of DNA and RNA with Absorption and
Fluorescence Spectroscopy
Sean R. Gallagher, UVP, LLC
Chapter 3 Reagent Preparation
Unit 3.1 Theoretical Discussion
Deb Chakravarti, Keck Graduate Institute
Unit 3.2 Practical Discussion
Deb Chakravarti, Keck Graduate Institute
Unit 3.3 Recipes
In-House
Chapter 4 Cell Culture Techniques
Unit 4.1 Aseptic Technique
Thomas Bykowski and Brian Stevenson, University of Kentucky
College of Medicine
Unit 4.2 Culture of Escherichia coli and Related
Bacteria
Sean P. Riley, Michael E. Woodman, and Brian Stevenson, University
of Kentucky College of Medicine
Chapter 5 Sample Preparation
Unit 5.1 Centrifugation and Cell Fractionation
In-House
Unit 5.2 Purification and Concentration of Nucleic
Acids
Dennis H. Dowhan, University of Queensland
Chapter 6 Chromatography
Unit 6.1 Overview of Chromatography
Buddhadeb Mallik, Bulbul Chakravarti, and Deb N. Chakravarti, Keck
Graduate Institute of Applied Life Sciences
Unit 6.2 Column Chromatography
Deb Chakravarti, Keck Graduate Institute
Chapter 7 Electrophoresis
Unit 7.1 Overview of Electrophoresis
Sean R. Gallagherr, UVP, LLC
Unit 7.2 Agarose Gel Electrophoresis
Jennifer A. Armstrong, The Claremont Colleges, and Joseph R.
Schulz, Occidental College
Unit 7.3 SDS-PAGE
Sean Gallagher, UVP, LLC
Unit 7.4 Staining of Gels
Sean Gallagher, UVP, LLC
Chapter 8 Blotting
Unit 8.1 Overview of Blotting
Maria Cristina Negritto, Pomona College, and Glenn M. Manthey,
City of Hope, Beckman Research Institute
Unit 8.2 Nucleic Acid Blotting: Southern and Northern
Laura L. Mays Hoopes, Pomona College
Unit 8.3 Protein Blotting: Immunoblotting
Sean Gallagher, UVP, LLC
Unit 8.4 Labeling DNA and Probe Preparation
Karl A. Haushalter, Harvey Mudd College
Chapter 9 Microscopy
Unit 9.1 Conventional Light Microscopy
Eric S. Cole, St. Olaf College
Unit 9.2 Immunofluorescence Microscopy
David J. Asai, Harvey Mudd College
Chapter 10 Enzymatic Reactions
Unit 10.1 Working with Enzymes
David Skrincosky and Cindy Santangelo, Worthington Biochemical
Corp.
Unit 10.2 Overview of PCR
Christine D. Kuslich, Molecular Profiling Institute, Buena Chui,
GE Healthcare, and Carl T. Yamashiro, Molecular Profiling
Institute
Unit 10.3 Real-Time PCR
Dean Fraga, College of Wooster, Tea Meulia, Ohio Agricultural
Research and Development Center, Steven Fenster, Ashland
University
Chapter 11 Bioinformatics Unit 11.1 Using NCBI Blast
Nicholas A. Stover, Bradley University, and Andre R.O. Cavalcanti,
Pomona College
Preface
Contributors List
Conversion Factors
Flow Chart: Application of Techniques
Emily Wiley, Claremont Colleges
Chapter 1 Volume/Weight Measurement
Unit 1.1 Volume Measurement
Andrew Zanella and Tom Davis, Claremont Colleges
Unit 1.2 Weight Measurement
Michael Guzy, Ohaus, Corp.
Chapter 2 Concentration Measurement
Unit 2.1 Spectrophotometry
Rob Morris, Ocean Optics, Inc.
Unit 2.2 Quantitation of DNA and RNA with Absorption and
Fluorescence Spectroscopy
Sean R. Gallagher, UVP, LLC
Chapter 3 Reagent Preparation
Unit 3.1 Theoretical Discussion
Deb Chakravarti, Keck Graduate Institute
Unit 3.2 Practical Discussion
Deb Chakravarti, Keck Graduate Institute
Unit 3.3 Recipes
In-House
Chapter 4 Cell Culture Techniques
Unit 4.1 Aseptic Technique
Thomas Bykowski and Brian Stevenson, University of Kentucky
College of Medicine
Unit 4.2 Culture of Escherichia coli and Related
Bacteria
Sean P. Riley, Michael E. Woodman, and Brian Stevenson, University
of Kentucky College of Medicine
Chapter 5 Sample Preparation
Unit 5.1 Centrifugation and Cell Fractionation
In-House
Unit 5.2 Purification and Concentration of Nucleic
Acids
Dennis H. Dowhan, University of Queensland
Chapter 6 Chromatography
Unit 6.1 Overview of Chromatography
Buddhadeb Mallik, Bulbul Chakravarti, and Deb N. Chakravarti, Keck
Graduate Institute of Applied Life Sciences
Unit 6.2 Column Chromatography
Deb Chakravarti, Keck Graduate Institute
Chapter 7 Electrophoresis
Unit 7.1 Overview of Electrophoresis
Sean R. Gallagherr, UVP, LLC
Unit 7.2 Agarose Gel Electrophoresis
Jennifer A. Armstrong, The Claremont Colleges, and Joseph R.
Schulz, Occidental College
Unit 7.3 SDS-PAGE
Sean Gallagher, UVP, LLC
Unit 7.4 Staining of Gels
Sean Gallagher, UVP, LLC
Chapter 8 Blotting
Unit 8.1 Overview of Blotting
Maria Cristina Negritto, Pomona College, and Glenn M. Manthey,
City of Hope, Beckman Research Institute
Unit 8.2 Nucleic Acid Blotting: Southern and Northern
Laura L. Mays Hoopes, Pomona College
Unit 8.3 Protein Blotting: Immunoblotting
Sean Gallagher, UVP, LLC
Unit 8.4 Labeling DNA and Probe Preparation
Karl A. Haushalter, Harvey Mudd College
Chapter 9 Microscopy
Unit 9.1 Conventional Light Microscopy
Eric S. Cole, St. Olaf College
Unit 9.2 Immunofluorescence Microscopy
David J. Asai, Harvey Mudd College
Chapter 10 Enzymatic Reactions
Unit 10.1 Working with Enzymes
David Skrincosky and Cindy Santangelo, Worthington Biochemical
Corp.
Unit 10.2 Overview of PCR
Christine D. Kuslich, Molecular Profiling Institute, Buena Chui,
GE Healthcare, and Carl T. Yamashiro, Molecular Profiling
Institute
Unit 10.3 Real-Time PCR
Dean Fraga, College of Wooster, Tea Meulia, Ohio Agricultural
Research and Development Center, Steven Fenster, Ashland
University
Chapter 11 Bioinformatics Unit 11.1 Using NCBI Blast
Nicholas A. Stover, Bradley University, and Andre R.O. Cavalcanti,
Pomona College
"Wow! This is really a great book for anyone learning
about or working in a research laboratory."
(Doody's, 30 August 2012)
about or working in a research laboratory."
(Doody's, 30 August 2012)
Sean R. Gallagher, Ph.D. is the Chief Technology Officer and Director of Product Development for UVP, Inc. (Upland, CA), where he oversees applied technology and product development for specialty light source and life science products. He is on the faculty of the Keck Graduate Institute of Applied Life Sciences and Harvey Mudd College (Claremont, CA) and holds eight patents on technology he developed. He received his doctorate in Botany from the University of California at Riverside and completed a post-doctoral position at Stanford University.
Emily A. Wiley, Ph.D. is an Associate Professor of Biology in the Joint Science Department of the Claremont-McKenna, Scripps, and Pitzer Colleges, Claremont, CA. She received her doctorate in Molecular Genetics from the University of Washington. She is actively involved in graduate and undergraduate teaching courses and is committed to providing proper training to those students. She has an active research program studying chromatin assembly in Tetrahymena thermophila.
Emily A. Wiley, Ph.D. is an Associate Professor of Biology in the Joint Science Department of the Claremont-McKenna, Scripps, and Pitzer Colleges, Claremont, CA. She received her doctorate in Molecular Genetics from the University of Washington. She is actively involved in graduate and undergraduate teaching courses and is committed to providing proper training to those students. She has an active research program studying chromatin assembly in Tetrahymena thermophila.
